glgB [2019-08-09 08:27:25]

1,4-alpha-glucan branching enzyme

locus: BSU_30980

pI: 6.24

mw: 73.49 kDa

protein length: 627 aa

gene length: 1884 bp

function: glycogen biosynthesis

product: 1,4-alpha-glucan branching enzyme

essential: no

ec: 2.4.1.18

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.5|Biosynthesis of glycogen]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 3,169,763 3,171,646

The protein

Catalyzed reaction/ biological activity

• Transfers a segment of a (14)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain (according to Swiss-Prot)

Protein family

• [SW|glycosyl hydrolase 13 family] (according to UniProt)

Paralogous protein(s)

• [protein|7D783DD180D950A4B030BF5EA78CCE930A466A07|AmyX]

Structure

• [PDB|5GQU] (from Cyanothece sp., 46% identity) [pubmed|28193843]

Expression and Regulation

Operons

• genes: [gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]-[gene|B665735B76462724A3C7A1E89D3E613C18F21399|glgC]-[gene|63A4FD35A2E72C665BFA3F2632F06C7A0C954087|glgD]-[gene|EBEA85AD543B79C321CB5F28D1B7E1248640DC1D|glgA]-[gene|3AB4513126EFF54996A54CB7DEB6062C1A06AAC3|glgP]
  description: [Pubmed|8145641]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|8145641], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8145641]

Biological materials

Mutant

• BKE30980 ([gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCATCCTTTCTGA, downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA
• BKK30980 ([gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCATCCTTTCTGA, downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA

References

• 8145641,28193843